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Past Events
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GNU SciTech Fest - 2019
The 28th February is celebrated as a National Science Day in India every year. I...
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Ganesh Chaturthi Celebrat...
Ganesh Chaturthi Celebration-2022
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Real Life Application of...
Real Life Application of Mathematics
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Synergy-2022
Synergy-2022
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Orienatation-2022
Orientation-2022
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Antrix-2022
Antrix-2022
Live Circulating Tumor Cell Isolation, Cultivation, and On-Chip 3D Organoids to Study Tumor Microenvironment

Description
In living tissue, cells exist in 3D microenvironments with intricate cell-cell and cell-matrix interactions and complex transport dynamics for nutrients and cells. Standard 2D, or monolayer, cell cultures are inadequate representations of this environment, which often makes them unreliable predictors of in vivo drug efficacy and toxicity. A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g., a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies.
3D spheroids more closely resemble in vivo tissue in terms of cellular communication and the development of extracellular matrices. These matrices help the cells to be able to move within their spheroid similar to the way cells would move in living tissue. The spheroids are thus improved models for cell migration, differentiation, survival, and growth. Furthermore, 3D cell cultures provide more accurate depiction of cell polarization, since in 2D, the cells can only be partially polarized. Moreover, cells grown in 3D exhibit different gene expression than those grown in 2D.
This event is organized to understand the new-age 3D cell culture technology based on nanotechnology and its application in understanding the tumor microenvironment for better treatment and management strategies, during which also highlight the relevance, to explore an opportunity for abroad research in the field of biomedical sciences.
The target audience were students, educators and researchers who have an interest in Science and Technology.
The webinar’s welcome address was delivered by Dr. Amit Parekh, Dean Science, Ganpat University and Principal MUIS.
The resource person explained the need to build 3D cell culture platform to mimic in vivo colon cancer tumor model and on-chip drug screening. Development of 3D cell culture chip to successfully culture different (Breast cancer, liver cancer, mouth cancer) tumor models on the same chip. The circulating tumor cell (CTC’s) detection and culturing, preparation of hydrogel-based blood vessels to mimic in vivo conditions. Further he also deliberated on the avenues in science and technology in Taiwan and the procedure to get admitted into higher education and doctoral programs in reputed universities.
The event was moderated by Dr. Bhushan B. Kulkarni.